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KMID : 0364219740170020081
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Korean Journal of Zoology
1974 Volume.17 No. 2 p.81 ~ p.92
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Studies on the Haemolymph Proteins during the Metamorphosis of the Pine Moth, Dendrolimus spectabilis Butler
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Yoo Chong-Myung
Lee Kyung-Ro
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Abstract
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The blood proteins of pine moth, Dendrolimus spectabilis Butler of different developmental stages were investigated by disc electrophoresis in acrylamide gels. Blood protein concentration was also determined during the metamorphosis. Protein concentration increased gradually with the growth of larva, reaching a maximum in themature larva, and the increase of protein bands also was accompanied. As the larva transforms into the prepupa the number of protein bands as well as the protein concentration dropped. A total of 22 bands were identified throughout the stages. Histochemical staining of the acrylamide gels by the PAS method, Toluidine blut O, and Sudan black demonstrated that the carbohydrate, mucopolysaccharide, and lipid were associated with certain blood proteins.
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